Member of Scientific Staff, Protein Production

Job description

LOCATION: King's Cross, London · PATTERN: Lab-based, full-time The opportunity We're a stealth startup in AI and bio, building infrastructure for data generation. The team is small and elite, the problems are hard, and the foundations are being laid right now. You'd help write them from the first line, with real ownership and a direct line to the founders. This role sits at the start of our protein work. Every measurement we make depends on the protein in the well being the right one, made the same way every time, and you would be one of the people making it. About us AI for biology has a data problem. The data that matters most doesn't exist yet, so we're building the infrastructure to produce it, with quality and traceability built in from day one. We're in stealth and heads-down on execution. We'll share more about what we're building once you've spoken with the team. The role You will make proteins, many of them at once. The work runs from DNA through to purified, quality-checked protein in 96-well format, ready for the binding and biophysical assays downstream of you. On the DNA side you will prepare expression templates from synthetic gene fragments, by PCR and by Golden Gate or Gibson assembly, and check them before they go forward. You will express proteins in cell-free systems first, with E. coli and mammalian (HEK293) expression following as the menu grows. You will purify them by affinity capture, mostly on magnetic beads in plates, and confirm what you made with quantification, CE-SDS and SEC. Every step is recorded as you go: what went in, which lot, which conditions, what came out, and what failed. Failed expressions and poor yields are kept as data, so they get the same care as the successes. As protocols settle you will help move them from the bench onto liquid handlers and our automated platform, working with the engineers who build it. This is a hands-on bench role, and in a small team you will also support the work either side of production when it needs doing. Downstream, that means molecular characterisation runs. Alongside, it means the cell culture behind our cell-based assays, from routine passage and banking to seeding assay plates. Your first 90 days FIRST 30 DAYS ◆ Run our core DNA preparation, cell-free expression and purification protocols to a consistent standard. ◆ Learn how a sample is tracked, from DNA order to purified prot…